non targeting control sinc  (Sangon Biotech)

Journal: Clinical Science (London, England : 1979)

Article Title: In vivo inhibition of TDO2 in fibroids results in widespread alteration in the tumor transcriptome

doi: 10.1042/CS20260395

Figure Lengend Snippet: Effects of TDO2 silencing in primary LSMC. Cells were transfected with siTDO2 or control siRNA (siNC) for 96 h, followed by assessment of gene expression by qRT-PCR. Expression levels of TDO2, VDR, MMP11, MMP14, COL11A1, CBX4, LINC02568, LINC01310, LINC02544, LINC02182, and miR-584-5p are shown. Data represent mean ± SEM from four independent experiments ( n = 4). Statistical significance is indicated as * P <0.05 and *** P <0.01.

Article Snippet: For gene silencing experiments, primary LSMCs were transfected with 50 nM of either a non-targeting control siRNA (siNC) or siRNA targeting TDO2 (siTDO2; 5′-CUAUCACUACCUGCGAUCAACUGUG-3′) using PureFection transfection reagent (System Biosciences, Mountain View, CA, U.S.A.), according to the manufacturer’s protocol.

Techniques: Transfection, Control, Gene Expression, Quantitative RT-PCR, Expressing

Journal: Poultry Science

Article Title: eIF3m promotes fowl adenovirus serotype 4 replication via interacting with ORF1B protein

doi: 10.1016/j.psj.2026.106566

Figure Lengend Snippet: Knockdown of eIF3m suppressed FAdV-4 replication in vitro. (A and B) LMH cells transfected with siRNA targeting at eIF3m or siNC as a negative control were collected at 24 h for qRT-PCR (A) and at 48 h for western blot (B) to evaluate the knockdown effect. (C and D) LMH cells transfected with 100nM/well siRNA or siNC were infected with CH/HNJZ/2015 at an MOI of 0.01. Western blot (C) and virus titration (D) were analyzed to investigate virus growth kinetics. The data shown represent the means±SD, and the experiments were repeated three times. The statistics analysis was performed by 2-way ANOVA. *, P < 0.05, **, P < 0.01.

Article Snippet: Based on the chicken eIF3m sequence obtained from GenBank, three siRNAs along with a non-targeting control (siNC) were synthesized by Shanghai Sangon Biotech.

Techniques: Knockdown, In Vitro, Transfection, Negative Control, Quantitative RT-PCR, Western Blot, Infection, Virus, Titration

Journal: Nature Communications

Article Title: SLC29A1 and SLC29A2 are human nicotinamide cell membrane transporters

doi: 10.1038/s41467-025-56402-y

Figure Lengend Snippet: a Structural similarity between nicotinamide and adenine, nicotinamide ribose and adenosine. b , c Accumulation of d 4 -NAM in AC16 cells ( b ) and MSCs ( c ) with or without inhibitors; NBTI, S-(4-Nitrobenzyl)−6-thioinosine; ADE, adenosine; NAM, normal nicotinamide without isotope labeling ( n = 3). d , e d 4 -NAM uptake in AC16 cells ( d ) and MSC ( e ) following transfection with non-targeting siRNA (siNC), siRNA targeting SLC29A1 (siENT1), SLC29A2 (siENT2) or both SLC29A1 and SLC29A2 (siENT1 + siENT2) ( n = 3). f d 4 -NAM uptake in CHO cells overexpressing SLC29A1 (ENT1), SLC29A2 (ENT2) or a vector control (vector) ( n = 3). g , h Close-up view of the binding pocket residues in close proximity to docked NAM within hENT1 ( g ) and hENT2 ( h ). i , j d 4 -NAM uptake by wild-type or point mutant of ENT1 ( i ) or ENT2 ( j ) point mutants ( n = 3). n represents biologically independent replicates. Data are represented as mean ± standard error of mean (s.e.m.). P values were calculated using one-way analysis of variance (ANOVA) followed by Dunnett’s multiple comparisons test for ( b , c ) Holm–Sidak’s multiple comparisons test for ( d , e , i , j ) and two-way ANOVA followed by Holm–Sidak’s multiple comparisons test for ( f ).

Article Snippet: siRNAs targeting human SLC29A1 , SLC29A2 , and non-targeting control (siNC) were obtained from Sangon Biotech (Shanghai, China) and resuspended at 20 μM in DEPC buffer.

Techniques: Quantitative Proteomics, Transfection, Plasmid Preparation, Control, Binding Assay, Mutagenesis